Gene expression analysis in mice with heart muscle-specific repression of CELF activity (MHC-CELFdelta)

ABSTRACT: Members of the CUG-BP, Elav-like family (CELF) regulate alternative splicing in the heart. In MHC-CELFdelta transgenic mice, CELF splicing activity is inhibited postnatally in heart muscle via expression of a nuclear dominant negative CELF protein under an a-myosin heavy chain promoter. MHC-CELFdelta mice develop dilated cardiomyopathy characterized by alternative splicing defects, enlarged hearts, and severe contractile dysfunction. In this study, gene expression profiles in the hearts of wild type, high- and low-expressing lines of MHC-CELFdelta mice were compared using microarrays. Gene ontology and pathway analyses identified contraction and calcium signaling as the most affected processes. Network analysis revealed that the serum response factor (SRF) network is highly affected.

ORGANISM(S): Mus musculus

SUBMITTER: Andrea Ladd 

PROVIDER: E-GEOD-40677 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress