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ChIPseq analysis of IRF4 and BATF in immune cells

ABSTRACT: Purpose: The purpose of this study is to find the binding partner of IRF4 in the context of Th17- cell differentiation. To this end, we have used ChIPseq analysis followed by de novo motif search around genome-wide binding sites to identify BATF as the binding partner for IRF4 in the context of not only Th17 cells but other immune cell types as well. Naïve T-cells isolated from the spleen of C57BL/6J mice are cultured under Th17, Th2 or Th0 polarizing conditions for 42 hrs and subject to ChIP using IRF4 and/or BATF antibodies followed by high-throughput sequencing. Bone marrow derived dendritic cells (BMDCs) were stimulated with LPS for 6hrs and similrly subjected to ChIPseq analysis with IRF4.

ORGANISM(S): Mus musculus

SUBMITTER: Smita Agrawal

PROVIDER: E-GEOD-40727 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A genomic regulatory element that directs assembly and function of immunespecific AP-1-IRF complexes

Project description:This SuperSeries is composed of the following subset Series: GSE40726: Transcriptional profiling of IRF4 -/- vs IRF4 +/- T-cells under Th17 polarizing conditions GSE40727: ChIPseq analysis of IRF4 and BATF in immune cells Refer to individual Series

2012-09-13 | E-GEOD-40483 | biostudies-arrayexpress

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