A user-friendly chromatographic method to purify small regulatory RNAs

ABSTRACT: The discovery of the small regulatory RNA populations has changed our vision of cellular regulations. Indeed, loaded on Argonaute proteins they formed ribonucleoprotein complexes that target complementary sequences and achieved widespread silencing mechanisms conserved in most eukaryotes. The recent development of deep sequencing approaches highly contributed to their detection. Small RNA isolation form cells and/or tissues remains a crucial stage to generate robust and relevant sequencing data. In 2006, a novel strategy based on anion-exchange chromatography has been purposed as an alternative to the standard size-isolation purification procedure. However, the eventual biases of such a method have been poorly investigated. Moreover, this strategy not only relies on advanced technical skil

ORGANISM(S): Drosophila melanogaster

SUBMITTER: SEVERINE CHAMBEYRON 

PROVIDER: E-GEOD-40748 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress