Tissue-specific regulation of chromatin insulator function

ABSTRACT: Chromatin insulators organize the genome into distinct transcriptional domains and contribute to cell type-specific chromatin organization. However, factors regulating tissue-specific insulator function have not yet been discovered. Here we identify the RNA recognition motif-containing protein, Shep, as a direct interactor of two individual components of the gypsy insulator complex in Drosophila. Mutation of shep improves gypsy-dependent enhancer blocking, indicating a role as a negative regulator of insulator activity. Unlike ubiquitously expressed core gypsy insulator proteins, Shep is highly expressed in the central nervous system (CNS) with lower expression in other tissues. We developed a novel, quantitative tissue-specific barrier assay to demonstrate that Shep functions as a negativ

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Ryan Dale 

PROVIDER: E-GEOD-40797 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress