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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Rotating wall vessel (RWV) grown A549s compared to monolayer grown A549s infected with F. tularensis SchuS4 over a 22 hour time-course (2, 6 and 22 h post infection)

ABSTRACT: A549 epithelial cells grown under two different conditions were compared. A549s grown in rotating wall vessel cultures display a more in-vivo like phenotype. The data here describe the transcriptional differences between the two culture methods when infected with Francisella tularensis SchuS4 over a 22 hour time-course 1 colour; Cy3 labelled, 6 biological replicates for each culture condition and time-point, A549s grown in RWVs (RWV) compared to A549 grown in monolayers (mono), Infected vs. NaM-CM-/ve

ORGANISM(S): Homo sapiens

SUBMITTER: Jonathan David

PROVIDER: E-GEOD-40808 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rotating wall vessel (RWV) grown A549s compared to monolayer grown A549s infected with F. tularensis SchuS4 over a 22 hour time-course (2, 6 and 22 h post infection)

Project description:A549 epithelial cells grown under two different conditions were compared. A549s grown in rotating wall vessel cultures display a more in-vivo like phenotype. The data here describe the transcriptional differences between the two culture methods when infected with Francisella tularensis SchuS4 over a 22 hour time-course

2012-12-01 | GSE40808 | GEO

Rotating wall vessel (RWV) grown A549s compared to monolayer grown A549s

Project description:A549 epithelial cells grown under two different conditions were compared. A549s grown in rotating wall vessel cultures disp[lay a more in-vivo like phenotype. The data here describe the transcriptional differences between the two culture methods 1 colour; Cy3 labelled, 5 biological replicates for each culture condition, A549s grown in RWVs (RWV) compared to A549 grown in monolayers (mono)

2012-10-01 | E-GEOD-36931 | biostudies-arrayexpress

Rotating wall vessel (RWV) grown A549s compared to monolayer grown A549s

2012-10-01 | GSE36931 | GEO

Transcription profiling of Francisella tularensis LVS wild type and hfq mutant strains grown on solid medium

Project description:Comparison of Francisella tularensis LVS wild-type and hfq mutant strains grown on chocolate agar plates.

2009-05-01 | E-MEXP-1884 | biostudies-arrayexpress

Transcription profiling of Francisella tularensis LVS wild type and hfq mutant strains grown on complex liquid medium

Project description:Comparison of Francisella tularensis LVS wild-type and hfq mutant strains grown in complex liquid broth.

2009-05-01 | E-MEXP-1891 | biostudies-arrayexpress

Transcription profiling of Francisella tularensis LVS wild type and hfq mutant strains grown on defined liquid medium (Chamberlains medium)

Project description:Comparison of Francisella tularensis LVS wild-type and hfq mutant strains grown in defined liquid medium (Chamberlain's medium)

2009-05-01 | E-MEXP-1892 | biostudies-arrayexpress

Gene expression data from F. tularensis-exposed neutrophils

Project description:We demonstrated recently that both constitutive and FAS-triggered apoptosis of human neutrophils are profoundly impaired by Francisella tularensis, but how this is achieved is largely unknown. To test the hypothesis that changes in neutrophil gene expression contribute to this phenotype, we used human oligonucleotide microarrays to identify differentially regulated genes in cells infected with F. tularensis strain LVS compared with uninfected controls. In order to examine the effect of F. tularensis on the neutrophil transcriptome, we performed microarray expression analysis on human neutrophils treated with F. tularensis subsp. holarctica live vaccine strain (LVS). Polymorphonuclear leukocytes (PMNs) were isolated from the blood of healthy donors. Control and F. tularensis-exposed PMNs were incubated at 37C for 0, 3, 6, 12, 24, and 48 hours.

2012-10-22 | E-GEOD-37416 | biostudies-arrayexpress

Francisella tularensis subsp. tularensis Induces a Unique Pulmonary Inflammatory Response: Role of Bacterial Gene Expression in Temporal Regulation of Host Defense Responses

Project description:Virulent Francisella tularensis induces a unique pulmonary inflammatory response characterized by temporal regulation of innate immune pathways correlating with altered bacterial gene expression patterns. The pulmonary transcriptional response to aerosolized virulent F. tularensis was compared to other lethal and non-lethal respiratory pathogens.

2013-04-15 | E-GEOD-44320 | biostudies-arrayexpress

Transcription profiling of Francisella tularensis LVS carrying plasmid pFNLTP6-gro or pFNLTP-gro-0851 (expressing rpoH) grown to mid exponential phase

Project description:F. tularensis LVS carrying plasmid pFNLTP6-gro or pFNLTP-gro-0851 (expressing rpoH) were grown to mid exponential phase in Schaedler + vitamin K3 medium and RNA isolated.

2009-07-01 | E-MEXP-2048 | biostudies-arrayexpress

Intra-macrophage Expression data of Francisella tularensis novicida U112

Project description:These samples are part of an experiment comparing the expression profiles of Francisella tularensis novicida grown in chemically defined medium and bacteria isolated 24 hours post infection of J774 macrophages to identify virulence factors Custom microarray submitted previously was used as the platform (GPL20119). The samples submitted here were compared with samples submitted previously (GSM1673555-57 in GSE68478) and differentially expressed genes during the intra-macrophage growth were identified.

2017-05-02 | E-GEOD-75689 | biostudies-arrayexpress

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