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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from mouse optic nerve head after optic nerve crush

ABSTRACT: Reactive gliosis is a complex process that involves profound changes in gene expression. We used microarray to indentify differentially expressed genes and to investigate the molecular mechanisms of reactive gliosis in optic nerve head in response to optic nerve crush injury. C57Bl/6 female mice were 6-8 weeks old at the time of optic nerve crush surgery. The optic nerve in the left eye was crush 1 mm behind the globe for 10 seconds and the right eye served as contralateral control. The animals were allowed to recover for 1 day, 3 day, 1 week, 3 weeks and 3 months before the optic nerve heads were collected. The naive control mice did not receive any surgery in either eye. Due to the small tissue size of the mouse optic nerve head, two optic nerve heads were pooled together for each microarray chip. The left eyes and the right eyes of two mice were combined respectively to form one pair of experiment and control samples. There were five biological replicates (10 mice) for each condition.

ORGANISM(S): Mus musculus

SUBMITTER: Tatjana Jakobs

PROVIDER: E-GEOD-40857 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The Time Course of Gene Expression during Reactive Gliosis in the Optic Nerve.

Qu Juan J Jakobs Tatjana C TC

PloS one 20130627 6

Reactive gliosis is a complex process that involves changes in gene expression and morphological remodeling. The mouse optic nerve, where astrocytes, microglia and oligodendrocytes interact with retinal ganglion cell axons and each other, is a particularly suitable model for studying the molecular mechanisms of reactive gliosis. We triggered gliosis at the mouse optic nerve head by retro orbital nerve crush. We followed the expression profiles of 14,000 genes from 1 day to 3 months, as the optic ...[more]

PMID: 23826199

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