Chd1 chromatin remodelers maintain nucleosome organization and repress cryptic transcription

ABSTRACT: Proper chromatin organization is essential for defining transcription units and maintaining genomic integrity in eukaryotes. Mutations affecting the chromatin structure can lead to increased cryptic transcription and genomic instability. In this study we found that deletion of the Schizosaccharomyces pombe Chd1-type chromatin remodelers, hrp1 and hrp3, causes strong, genome-wide accumulation of antisense transcripts, while the amount of coding mRNA transcripts is mostly unaffected. Nucleosome mapping revealed a specific role for Chd1-remodelers in the positioning of nucleosomes in gene coding regions. While the arrangement of nucleosomes in promoter regions was similar to WT, nucleosome organization within coding regions was remarkably irregular in hrp1M-bM-^HM-^Fhrp3M-bM-^HM-^F strain. We

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Tamas Fischer 

PROVIDER: E-GEOD-40872 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress