The Chlamydia trachomatis plasmid-encoded Pgp4 is a transcriptional regulator of virulence associated chromosomal genes

ABSTRACT: Chlamydia trachomatis causes chronic inflammatory diseases of the eye and genital tract of global medical importance. The chlamydial plasmid plays an important role in the pathophysiology of these diseases as plasmid-deficient organisms are highly attenuated. The plasmid encodes both noncoding RNAs and eight conserved ORFs of undefined function. To understand plasmid gene function we generated plasmid shuttle vectors with deletions in each of the eight ORFs. The individual deletion mutants were used to transform chlamydiae and the transformants were characterized in terms of plasmid biology and transcriptional profiling. We show that pgp1-2, -6 and -8 are essential for plasmid maintenance while the other ORFs can be deleted and the plasmid stably maintained. We further show that a pgp

ORGANISM(S): Chlamydia trachomatis

SUBMITTER: Dan Sturdevant 

PROVIDER: E-GEOD-40979 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress