CHD1 remodelers space nucleosomes in vitro and link regular arrays to 5M-bM-^@M-^Y ends of genes in S. Pombe

ABSTRACT: Nucleosome positioning governs access to eukaryotic genomes. Many genes show a stereotypic organisation at their 5M-bM-^@M-^Y end: a nucleosome free region just upstream of the transcription start site (TSS) followed by a regular nucleosomal array over the coding region. The determinants for this pervasive pattern are unclear, but nucleosome remodeling ATPases likely are critical. Now we employ deletion mutants to study the role of nucleosome remodeling ATPases in global nucleosome positioning in S. pombe and the corresponding changes in expression patterns. We find a striking evolutionary shift in remodeling enzyme usage between budding and fission yeast. The S. pombe RSC remodeling complex seems not involved in nucleosome positioning, despite its prominent role in S. cerevisiae. While la

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Karl Ekwall 

PROVIDER: E-GEOD-41024 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress