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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of ischemia-reperfused Rattus norvegicus heart tissue samples

ABSTRACT: Rats underwent surgery for LAD ligation for 30 min followed by reperfusion. Heart ventricles were collected 2d or 7d after reperfusion. Experiment Overall Design: rats were divided in following groups that underwent LAD occlusion or not (SHAM): Experiment Overall Design: 1. 7d-IR (n=3) Experiment Overall Design: 2. 7d-sham (n=3) Experiment Overall Design: 3. 2d-IR (n=3) Experiment Overall Design: 4. 7d-sham (n=3)

ORGANISM(S): Rattus norvegicus

DISEASE(S): normal

SUBMITTER: Sashwati Roy

PROVIDER: E-GEOD-4105 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analysis of the ischemia-reperfused remodeling myocardium: temporal changes in inflammation and extracellular matrix.

Roy Sashwati S Khanna Savita S Kuhn Donald E DE Rink Cameron C Williams Willis T WT Zweier Jay L JL Sen Chandan K CK

Physiological genomics 20060322 3

cDNA microarray analysis was performed to screen 15,000 genes and expressed sequence tags (ESTs) to identify changes in the ischemia-reperfused (I-R) rat myocardial transcriptome in the early (day 2) and late (day 7) inflammatory phases of acute myocardial infarction. Lists of candidate genes that were affected by I-R transiently (2 or 7 days only) or on a more sustained basis (2 and 7 days) were derived. The candidate genes represented three major functional categories: extracellular matrix, ap ...[more]

PMID: 16554547

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Rat LAD IR study

Project description:Rats underwent surgery for LAD ligation for 30 min followed by reperfusion. Heart ventricles were collected 2d or 7d after reperfusion. Keywords: rat heart ventricles, LAD - left anterior descending coronary artery, IR - ischemia-reperfusion

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Potential Target Genes of MicroRNA-21 In Ischemia-Reperfusion Injury Of The Gracilis Muscle In Rats

Project description:Transcriptional profiling of rat gracilis muscle after ischemia 4h and reperfusion for 1d, 3d, 7d and 14d v.s. sham control, and correlate the downregulated transcripts to the computational predicted gene targets of ron-miR-21 Following 4 h of ischemia and subsequent reperfusion for 4 h of the gracilis muscles, three miRNAs (miR-21, miR-200c, and miR-205) of 350 tested rat miRNAs were found to have an increased expression in the miRNA array.The expression of the mRNA in the muscle specimens after 4 h of ischemia and reperfusion for 1, 3, 7, and 14 d were detected with the Agilent Whole Rat Genome 4 Ã 44 k oligo microarray. A combined approach using a computational prediction algorithm that included miRanda, PicTar, TargetScanS, MirTarget2, RNAhybrid, and the whole genome microarray experiment was performed by monitoring the mRNA:miRNA association to identify potential target genes. Four-condition experiment, Gracilis muscle after ischemia-reperfusion injury for 1d, 3d, 7d and 14d v.s.Gracilis muscle (sham control), Biological replicates: 1 control replicate, 1 experiement replicate (each condition).

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2008-06-15 | E-GEOD-6730 | biostudies-arrayexpress

phosphoproteome of hippocampus after focal cerebral ischemia 1.5 h and reperfusion 24 h in mice

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