The APSES Transcription factor Efg1 regulates a novel phenotype switch in Candida parapsilosis [RNA-seq].

ABSTRACT: In Candida albicans the Efg1 transcription factor (a member of the APSES family) is an important regulator of hyphal growth, and of the white-to-opaque transition. In contrast, we show that the Efg1 ortholog in Candida parapsilosis is a major regulator of a different morphological switch at the colony level, from a concentric to smooth morphology. The rate of switching is at least 100-fold increased in an efg1 knockout relative to wild type. Deleting efg1 also reduces biofilm formation, and results in increased sensitivity to SDS, Congo red, caspofungin and calcofluor white in cells of both morphologies. Biofilm reduction is more dramatic in in vitro than in in vivo models. We use ChIP-seq to show that Efg1 binds to 502 promoter regions, including 70 potential transcription factors or regu

ORGANISM(S): Candida parapsilosis

SUBMITTER: Geraldine Butler 

PROVIDER: E-GEOD-41064 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress