Transcript analyses of cisplatin and Sky1 effects in Saccharomyces cerevisiae

ABSTRACT: Sky1 is a Saccharomyces cerevisiae rich serine-arginine (SR) protein-specific kinase and its enzymatic activity is essential in the cytotoxicity caused by cisplatin, although the molecular mechanisms supporting this function are not understood. We present a transcriptome analysis discriminating between RNA changes induced by cisplatin which are dependent or independent of the Sky1 function. The S. cerevisiae strain W303 (MATa ade2-1 can1-100 leu2-3,112 trp1-100 ura3- 52) and its derivative W303-M-NM-^Tsky1 previously described (RodrM-CM--guez- Lombardero et al., 2012) have been used in these analyses. Biological replicates of cultures and treatments were run in triplicate. The yeast cells were pre-cultured over night in 10mL of complete synthetic medium (SD) prepared as previously describ

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: M Esperanza Cerdan 

PROVIDER: E-GEOD-41094 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress