Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Comparison of transcriptome profile between wild-type and dao mutant plants

ABSTRACT: We identified and characterized that the rice Dioxygenase for Auxin Oxidation (dao) mutant displays pleiotropic phenotypes, including indehiscent anthers, sterile pollens, and increased free IAA levels in anthers.We conclude that DAO-mediated auxin catabolism is essential for auxin homeostasis and later stages of plant reproductive development, including anther dehiscence, and pollen viability. We used microarrays to identify differentially expressed genes in dao. For genome-wide expression analysis of dao, three replicates of WT and dao samples (RNA from mature anthers) were analyzed on Affymetrix Genechip® Rice Genome arrays by an Affymetrix service facility (CapitalBio Corporation) according to the manufacturer’s protocols. Genes showing a 2-fold change with a q-value ≤ 0.05 were considered to be differentially expressed.

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: Zhao Gang 

PROVIDER: E-GEOD-41097 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Indole-3-acetic acid (IAA), the natural auxin in plants, regulates many aspects of plant growth and development. Extensive analyses have elucidated the components of auxin biosynthesis, transport, and signaling, but the physiological roles and molecular mechanisms of auxin degradation remain elusive. Here, we demonstrate that the dioxygenase for auxin oxidation (DAO) gene, encoding a putative 2-oxoglutarate-dependent-Fe (II) dioxygenase, is essential for anther dehiscence, pollen fertility, and  ...[more]

Similar Datasets

Transcriptomics Comparison of transcriptome profile between wild-type and dao mutant plants
2012-09-25 | GSE41097 | GEO
Transcriptomics miR167 limits anther growth to potentiate anther dehiscence
2019-07-15 | GSE122436 | GEO
Transcriptomics Auxin-induced gene expression in mesocotyl elongation of maize inbred line 3681-4 tolerant to deep-sowing
2009-03-26 | E-GEOD-15371 | biostudies-arrayexpress
Transcriptomics Analysis of the effect of auxin biosynthesis inhibitor L-AOPP on the gene expression profile (shoot apical meristem)
2015-12-31 | E-GEOD-30092 | biostudies-arrayexpress
Transcriptomics A role of DAO1 in oxidation of IAA amino acid conjugates revealed through metabolite, high throughput transcript and protein level analysis
2020-10-30 | GSE160438 | GEO
Transcriptomics Studies of a rice sterile mutant sstl
2019-04-15 | GSE129579 | GEO
Transcriptomics Transcription profiling of hy5 single mutant, hyh single mutant, hy5 hyh double mutant and wild type Arabidopsis seedlings of two different ecotypes and with or without auxin treatment
2006-11-08 | E-MEXP-715 | biostudies-arrayexpress
Proteomics Variation in auxin sensing guides AUX/IAA repressor ubiquitylation and destruction
2017-06-14 | PXD004027 | Pride
Genomics miR396-OsGRF6 module controls rice inflorescence architecture
2016-05-13 | E-GEOD-72694 | biostudies-arrayexpress
Transcriptomics Comparison of transcriptome profile between wild-type and Epi-df mutant plants
2012-07-12 | E-GEOD-39298 | biostudies-arrayexpress