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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of bone marrow-derived macrophages from SOCS3+/+ and SOCS3-/- mice to study the effects of SOCS3 on the transcriptional responses of the macrophages to IL-6.

ABSTRACT: Effects of SOCS3 on the transcriptional response of bone marrow-derived macrophages to IL-6. Fetal liver cells from SOCS3+/+ or SOCS3-/- embryos were used to reconstitute recipient mice. Donor derived bone marrow from these mice was differentiated to macrophages. Macrophages were either unstimulated, or stimulated for 100 or 400 minutes with 10 ng/ml IL-6. Keywords = macrophage, SOCS3, IL-6, interferon

ORGANISM(S): Mus musculus

SUBMITTER: Roland Lang

PROVIDER: E-GEOD-411 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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SOCS3 regulates the plasticity of gp130 signaling.

Lang Roland R Pauleau Anne-Laure AL Parganas Evan E Takahashi Yutaka Y Mages Jörg J Ihle James N JN Rutschman Robert R Murray Peter J PJ

Nature immunology 20030518 6

Suppressor of cytokine signaling (SOCS) proteins are feedback inhibitors of the Janus kinase (JAK) and signal transducer and activator of transcription (STAT) signaling pathway. SOCS3 is upregulated by several signals in macrophages and has been implicated as a regulator of various signaling pathways. Here we show that phosphorylation of STAT3 is prolonged in mouse Socs3-deficient macrophages after stimulation with interleukin-6 (IL-6) but not IL-10, indicating that SOCS3 specifically affects si ...[more]

PMID: 12754506

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