Nucleosome remodeling by the SWI/SNF complex in non-coding RNA-mediated transcriptional gene silencing (ChIP-seq)

ABSTRACT: RNA-mediated transcriptional silencing prevents deleterious effects of transposon activity and controls the expression of protein-coding genes. It involves long non-coding RNAs (lncRNAs)1, which in Arabidopsis thaliana are produced by a specialized RNA Polymerase V (Pol V)2. lncRNAs guide Argonaute-siRNA complexes to specific genomic loci and mediate the establishment of DNA methylation3,4. The mechanism by which lncRNAs affect chromatin structure and mRNA production remains mostly unknown. Here we identify the SWI/SNF nucleosome remodeling complex as a component of the RNA-mediated transcriptional silencing pathway. We found that SWI3, an essential subunit of the SWI/SNF complex, physically interacts with a lncRNA-binding IDN2 protein5,6. RNA-mediated DNA methylation and transcriptional s

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: M Rowley 

PROVIDER: E-GEOD-41143 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress