Lymphoid progenitor gene expression in Notch1 activated Gfi1-deleted cells

ABSTRACT: Growth factor independent 1 (Gfi1) is a transcriptional repressor originally identified as a common integration site in Moloney-murine-leukemia-virus-induced T-cell leukemia. Gfi1-/- mice display increased apoptosis of developing thymocytes and T lymphopenia; however, there are contradictory reports of the absolute number of Gfi1-/- early T lineage progenitors. We used floxed alleles of Gfi1 crossed to various T-cell-specific Cre transgenes to map the requirements for Gfi1 during lymphoid priming and development. We show that Gfi1 is necessary for the proper formation and function of both lymphoid-primed multipotent progenitors and early T lineage progenitors. These defects correlate with a global inability of Gfi1-/- progenitors to enforce the activation of lymphoid genes including IL7R

ORGANISM(S): Mus musculus

SUBMITTER: H. Leighton Grimes 

PROVIDER: E-GEOD-41162 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress