Mechanistic analysis of the model obesogen Tributyltin in the 3T3-L1 cell line

ABSTRACT: Obesogenic compounds are chemicals that might have an influence on obesity development through in utero or chronic lifetime exposure. Tributyltin (TBT) is one of the most studied obesogenic compounds, inducing adipogenesis in vitro and increasing body fat after in utero exposure of mice. This study was designed to unravel the molecular mechanisms of TBT, using microarray analysis, and to evaluate the use of toxicogenomics for obesogen screening. The murine 3T3-L1 cell line was used to study TBT induced adipogenesis. Lipid staining as well as gene expression measurements of an adipocyte specific marker gene were performed to select relevant concentrations (10 nM, 50 nM) and time points (day1, day10) for microarray analysis. Additionally, solvent control and positive control (MDI) treated 3T

ORGANISM(S): Mus musculus

SUBMITTER: Anna Pereira-Fernandes 

PROVIDER: E-GEOD-41340 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress