Transcription profiling of human breast cancer cells NF639 cells original population vs EGCG-resistant population

ABSTRACT: Overexpression of the epidermal growth factor receptor family member Her-2/neu in breast cancer leads to autophosphorylation of the receptor, and induction of multiple downstream signaling pathways including Akt kinase to NF-B cascade that is associated with poor prognosis. Previously, we demonstrated green tea polyhenol epigallocatechin 3-gallate (EGCG) inhibits growth of NF639 Her-2/neu-driven breast cancer cells via reducing receptor autophosphorylation, and downstream Akt and NF-B activities (Pianetti et al., 2002). Interestingly, we noted that upon prolonged culture in the presence of EGCG some cells developed resistance to the polyphenol. Here we report that resistant cells have lost tyrosine phosphorylation on the Her-2/neu receptor. Surprisingly, they displayed elevated NF-ï

ORGANISM(S): Mus musculus

SUBMITTER: Gail Sonenshein 

PROVIDER: E-GEOD-4140 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress