Zic3 interacts with distant regulatory elements to regulate zebrafish developmental genes

ABSTRACT: Zic3 regulates early embryonic patterning in vertebrates. Its loss-of-function disrupts gastrulation, left-right patterning, and neurogenesis. We use the zebrafish as a model to study the developmental role of Zic3 in vivo. Using a combination of two genomics approaches – ChIP-seq and microarray, we identified Zic3 targets, which include genes from the Nodal and Wnt pathways, and show for the first time cis-regulation of these genes by Zic3 using in vivo enhancer assay. We uncovered a previously unrecognized link between Zic3 and the non-canonical Wnt pathway in gastrulation and left-right patterning, and identified neural pre-pattern genes as Zic3 targets during the early steps of neural induction. Zic3 preferably binds to distal intergenic regions, some of which contain evolutionarily co

ORGANISM(S): Danio rerio

SUBMITTER: Vibhor Kumar 

PROVIDER: E-GEOD-41458 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress