Nucleosome driven transcription factor binding and gene regulation [ChIP-Seq]

ABSTRACT: Elucidating the global function of a transcription factor implies the identification of its target genes and genomic binding sites. The role of chromatin in this context is unclear, but the dominant view is that factors bind preferentially to nucleosome-depleted regions, identified as DNaseI-hypersensitive sites (DHS). Here we show by chromatin-IP, MNase and DNaseI assays followed by deep sequencing that the progesterone receptor (PR) requires nucleosomes for optimal binding and function. In breast cancer cells treated with progestins we identified 25,000 PR binding sites (PRbs), the majority encompassing several copies of the hexanucleotide TGTYCY, highly abundant in the genome. We found that functional PRbs accumulate around progesterone-induced genes mainly in enhancers, are enriched in

ORGANISM(S): Homo sapiens

SUBMITTER: Miguel Beato 

PROVIDER: E-GEOD-41466 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress