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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of models of cardiac remodelling: myocardial infarction or transverse aortic constriction in left ventricle free wall and septum

ABSTRACT: We employed the Affymetrix GeneChip technology to evaluate the patterns of expression in two different in vivo models of cardiac remodeling and in two different regions (left ventricle free wall and septum) of the heart. Mice underwent transverse aortic constriction (TAC); myocardial infarction (MI) or Sham operation and RNA from the left ventricle free wall and the septum was isolated 1 week later.

ORGANISM(S): Mus musculus

SUBMITTER: Maria Mirotsou

PROVIDER: E-GEOD-415 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Elucidating the molecular mechanism of cardiac remodeling using a comparative genomic approach.

Mirotsou Maria M Watanabe Coran M H CM Schultz Peter G PG Pratt Richard E RE Dzau Victor J VJ

Physiological genomics 20031017 2

It is proposed that analysis of global gene expression would provide an understanding of the molecular mechanisms of cardiac remodeling. However, previous studies have only provided "snapshots" of differential gene expression. Furthermore, the differences in gene expression between regions of the heart that can result in sampling variability have not been characterized. In this study, we employed the Affymetrix GeneChip technology to evaluate the patterns of expression in two different in vivo m ...[more]

PMID: 12902547

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Mouse models of cardiac remodeling

Project description:We employed the Affymetrix GeneChip technology to evaluate the patterns of expression in two different in vivo models of cardiac remodeling and in two different regions (left ventricle free wall and septum) of the heart. Mice underwent transverse aortic constriction (TAC); myocardial infarction (MI) or Sham operation and RNA from the left ventricle free wall and the septum was isolated 1 week later. Keywords: other

2003-08-22 | GSE415 | GEO

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Effect of ~ 25 days cortisol treatment on late gestation fetal left ventricle

Project description:We have previously shown in sheep that 10 days of modest chronic increase in maternal cortisol result in fetal heart enlargement and Purkinje cell apoptosis. In subsequent studies in which we extended the duration of cortisol infusion (1mg/kg/d) to term, we found a dramatic incidence of stillbirth in the pregnancies with chronically increased cortisol and associated maternal hyperglycemia. In previous studies of the intraventricular septum from these fetuses we found significantly differentially regulated genes in the term fetuses (ie after ~25 days of cortisol) in pathways consistent with altered metabolism in the heart, particularly in mitochondria, associated with responses to hypoxia and to nutrient. Analysis of mitochondrial number by quantitative real-time PCR confirmed a significant decrease. We extended this investigation to the left ventricular free wall of these fetuses.le. Fewer genes were differentially regulated in left ventricle in the near term fetuses. In the LV of this cohort of fetuses, the nonredundant KEGG pathways represented by the DEG were insulin resistance and adipocytokine signaling pathway. GSEA analysis of the DEG in LV identified metabolic pathways as the nonredundant pathway altered. Comparison of the change in gene expression in biceps to that in cardiac intraventricular septum and left ventricle showed few common genes with little overlap in specific metabolic or signaling pathways, despite effects on mitochondria and metabolism in both heart and biceps. Our results suggest that glucocorticoid exposure affects nuclear genes important to mitochondrial activity and reactive oxygen in both cardiac and skeletal muscle tissues in a tissue specific manner.

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Expression data from murine cardiac fibroblasts and endothelial cells following Transverse Aortic Constriction

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Heart ventricles proteomic profile in pulmonary arterial hypertension

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