Highly parallel assays of tissue-specific enhancers in whole Drosophila embryos

ABSTRACT: Understanding transcriptional regulatory networks requires the identification and characterization of cis-regulatory modules (CRMs), DNA sequences which can direct expression of associated genes to a specific cell type and/or developmental stage. Reporter assays for the capacity of a candidate CRM to activate a heterologous promoter have been productive but suffer limited throughput or fail to convey information on cell type specificity. We have developed an assay in which reporter constructs containing a pool of candidate CRMs are introduced in parallel to Drosophila embryos along with a common cell-type-specific second marker; candidate CRMs isolated by PCR from FACS-purified double-positive cells can by quantitated by high-throughput sequencing, and their relative abundance compared t

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Luis Barrera 

PROVIDER: E-GEOD-41503 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress