Integrated analysis of transcript level regulation of metabolism during human adipocyte differentiation [miR]

ABSTRACT: Here, we have focused on studying the link between metabolic changes driven by the differentiation into mature adipocytes of a human preadipocyte cell line (SGBS) and their regulation, through a combined experimental and computational approach. By collecting data on gene expression, PPARg, CEBPa, LXR and H3K4me3 genome-wide ChIP-seq profles and transcriptome-wide microRNA target identification for miR-27a, miR29a and miR-222, and using constraint-based modeling to estimate metabolic reaction activity, we obtained a comprehensive set of information highlighting how epigenetic, transcriptional and post-transcriptional regulation impacts the metabolic network. Illumina HT12 V4.0 microarrays: Transcriptome analysis 24 hours following overexpression of indicated microRNAs or control siRNAs on i

ORGANISM(S): Homo sapiens

SUBMITTER: Merja Heinäniemi 

PROVIDER: E-GEOD-41576 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress