Spatial Compartmentalization at the Nuclear Periphery Characterized by Genome-wide Mapping

ABSTRACT: How gene positioning to the nuclear periphery regulates transcription remains largely unclear. We have previously observed the differential compartmentalization of transcription factors and histone modifications at the nuclear periphery in mouse C2C12 myoblasts. Here, we have integrated high throughput DNA sequencing into the DNA adenine methyltransferase identification (DamID) assay, and have identified ~15, 000 sequencing-based Lamina-Associated Domains (sLADs) in mouse 3T3 fibroblasts and C2C12 myoblasts. These genomic regions range from a few kb to over 1 Mb and cover ~30% of the genome, and are spatially proximal to the nuclear lamina (NL). Active histone modifications such as H3K4me2, H3K9Ac, H3K36me3 and H3K79me2 are all localized away from the nuclear periphery microscopically, and

ORGANISM(S): Mus musculus

SUBMITTER: Feinan Wu 

PROVIDER: E-GEOD-41583 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress