Pitx1 broadly associates with limb enhancers and is enriched on hindlimb cis-regulatory elements

ABSTRACT: Extensive functional analyses have demonstrated that the pituitary homeodomain transcription factor Pitx1 plays a critical role in specifying hindlimb morphology in vertebrates. However, much less is known regarding the target genes and cis-regulatory elements through which Pitx1 acts. Earlier studies suggested that the hindlimb transcription factors Tbx4, HoxC10, and HoxC11 might be transcriptional targets of Pitx1, but definitive evidence for direct regulatory interactions has been lacking. Using ChIP-Seq on embryonic mouse hindlimbs, we have pinpointed the genome-wide location of Pitx1 binding sites during mouse hindlimb development and identified potential gene targets for Pitx1. We determined that Pitx1 binding is significantly enriched near genes involved in limb morphogenesis, inclu

ORGANISM(S): Mus musculus

SUBMITTER: Carlos Infante 

PROVIDER: E-GEOD-41591 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress