RNAi triggered by specialized machinery silences developmental genes and retrotransposons (ChIP-chip)

ABSTRACT: RNAi is a conserved mechanism in which small interfering RNAs (siRNAs) guide the degradation of cognate RNAs, but also promote heterochromatin assembly at repetitive DNA elements such as centromeric repeats. However, the full extent of RNAi functions and its endogenous targets have not been explored. Here we show that in the fission yeast Schizosaccharomyces pombe, RNAi and heterochromatin factors cooperate to silence diverse loci, including sexual differentiation genes, genes encoding transmembrane proteins, and retrotransposons that are also targeted by the exosome RNA degradation machinery. In the absence of the exosome, transcripts are processed preferentially by the RNAi machinery, revealing widespread occurrence of siRNA clusters and corresponding increase in heterochromatin modifica

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Shiv Grewal 

PROVIDER: E-GEOD-41642 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress