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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Disulphide bond inhibitors in 2 cancer cell lines

ABSTRACT: To elucidate which ER cargo maturation processes may be oxygen dependent, we first reasoned that the transcriptional response may be tailored specifically to counteract the mechanism of the imposed stress. Therefore, we compared the transcriptional regulation of the ERome during anoxic conditions to ER stress caused by lack of glycosylation (tunicamycin) or chaperone inactivation by calcium depletion (thapsigargin) In two cancer cell lines (colon carcinoma HCT116, and hepatocellular carcinoma Hep G2) cells were given one of 4 treatments. 24 hours of 0.0% O2 (Anoxia), 1.5 µg/ml tunicamycin or 0.3 µM thapsigargin with a control 21% O2 (Normoxia). Cells were exposed to hypoxia and anoxia in H35 and H85 HypOxystations (Don Whitley Scientific). 3 biological replicates of each experiment were carried out. Total of 24 samples.

ORGANISM(S): Homo sapiens

SUBMITTER: Nicholas Harding

PROVIDER: E-GEOD-41666 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Secretome characterization of nucleus pulposus (NP) cells subjected to ER stress

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2020-12-03 | PXD019151 | Pride

ER stress impairs the insulin signaling pathway through mitochondrial damage in SH-SY5Y human neuroblastoma cells (part 1)

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Experimental Selection for Drosophila Survival in Extremely Low O2 Environments

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Disulphide bond inhibitors in 2 cancer cell lines

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Whole transcriptome profiles and ribosome profiling of thapsigargin- and tunicamycin-treated LN308 cells

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2013-04-06 | E-GEOD-45821 | biostudies-arrayexpress

A miR-494 dependent feedback loop regulates ER stress (ANAN-376 Project)

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2021-09-09 | PXD019992 | Pride

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