Different levels of canonical Wnt signaling exert distinct roles in the colonic epithelium

ABSTRACT: There is a gradient of M-NM-2-catenin expression along the colonic crypt axis with the highest levels at the crypt bottom. However, it remains unclear whether different levels of canonical Wnt signaling exert distinct roles in the colonic epithelium. In the present study, we first showed that the canonical Wnt signaling is active in the proliferative compartment of normal colonic crypts by separating actively proliferating progenitor cells from non-proliferating cells in the colon using transgenic mice expressing a histone H2B-green fluorescent protein (GFP) fusion protein under the control of a tetracycline responsive regulatory element. Subsequently, we investigated the dose-dependent effect of canonical Wnt activation on colonic epithelial differentiation by controlling the expression l

ORGANISM(S): Mus musculus

SUBMITTER: Yasuhiro Yamada 

PROVIDER: E-GEOD-41688 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress