Expression profiles in testis of mouse inter-subspecific hybrids

ABSTRACT: According to Dobzhansky-Muller model, hybrid sterility is a consequence of independent evolution of related taxa resulting in incompatible interaction during gametogenesis of their hybrids. We proposed that asynapsis of heterospecific chromosomes in meiotic prophase provides a general and recurrently evolving trigger for the meiotic arrest of interspecific F1 hybrids. We used genome-wide expression profiling to quantify misexpression of Chr X and Chr Y genes. The total RNA (20M-bM-^@M-^S30 ng) samples were extracted from 14.5 days juvenile testes (pool of 4 samples), converted to cDNA using the Affymetrix 3 IVT Express Kit and hybridized to Affymetrix Mouse Gene 1.0ST GeneChips (core facility of the Institute of Molecular Genetics AS CR, Prague, Czech Rep.) We visually checked intensity

ORGANISM(S): Mus musculus

SUBMITTER: Petr Simecek 

PROVIDER: E-GEOD-41707 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress