Genome-Scale Identification of Membrane-Associated Human mRNAs

ABSTRACT: The subcellular localization of proteins is critical to their biological roles. Moreover, whether a protein is membrane-bound, secreted, or intracellular affects the usefulness of, and the strategies for, using a protein as a diagnostic marker or a target for therapy. We employed a rapid and efficient experimental approach to classify thousands of human gene products as either membrane-associated/secreted (MS) or cytosolic/nuclear (CN). Using subcellular fractionation methods, we separated mRNAs associated with membranes from those associated with the soluble cytosolic fraction and analyzed these two pools by comparative hybridization to DNA microarrays. Analysis of 11 different human cell lines, representing lymphoid, myeloid, breast, ovarian, hepatic, colon, and prostate tissues, identif

ORGANISM(S): Homo sapiens

SUBMITTER: Max Diehn 

PROVIDER: E-GEOD-4175 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress