Accelerated high-yield generation of limb-innervating motor neurons from human stem cells

ABSTRACT: Human pluripotent stem cells are a promising source of diverse cells for developmental studies, cell transplantation, disease modeling, and drug testing. However, their widespread use even for intensely studied cell types like spinal motor neurons, is hindered by the long duration and low yields of existing protocols for in vitro differentiation and by the molecular heterogeneity of the populations generated. We report a combination of small molecules that induce up to 50% motor neurons within 3 weeks from human pluripotent stem cells with defined subtype identities that are relevant to neurodegenerative diseases. Despite their accelerated differentiation, motor neurons expressed combinations of HB9, ISL1 and column-specific markers that mirror those observed in vivo in human fetal spina

ORGANISM(S): Homo sapiens

SUBMITTER: Mackenzie Amoroso 

PROVIDER: E-GEOD-41795 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress