Expression profiles of primary bone marrow derived mouse macrophages M-bM-^@M-^S untreated and treated with LPS or LPS+PGE

ABSTRACT: The polarization of macrophages into an anti-inflammatory or regulatory phenotype plays an important role in resolving inflammation. PGE2 regulates macrophage polarization via a PKA dependent pathway. PKA phosphorylates SIKs, inhibiting their ability to phosphorylate CRTC3 in cells. This in turn allows CRTC3 to translocate to the nucleus where it acts as a co-activator with the transcription factor CREB to induce IL-10 transcription. In line with this we find that either genetic or pharmacological inhibition of SIKs mimics the effect of PGE2 on IL-10 production. We show here that PGE2, in combination with LPS, is able to promote a regulatory like phenotype in macrophages characterized by high expression of IL-10 as well as the regulatory markers SPHK1 and LIGHT. Gene expression profil

ORGANISM(S): Mus musculus

SUBMITTER: Manikhandan Mudaliar 

PROVIDER: E-GEOD-41833 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress