Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Real-time quantitative PCR analysis of microRNA in keratinocytes and reconstituted epidermis

ABSTRACT: Analysis of microRNA expression in keratinocytes by relative quantification (RQ) using the comparative Ct method. Real-time quantitative PCR analysis of microRNA in keratinocytes or reconstituted epidermis exposed to IL-22, LPS or UV radiation or a small molecule drug. Keratinocytes or reconstituted human epidermis were exposed to indicated treatments. A qPCR analysis of 365 microRNAs in keratinocytes. RNA (20 ng per reaction) reverse transcribed using 8 x Multiplex RT pools. Each resulting cDNA pool was mixed with TaqMan Universal PCR Master Mix and loaded in corresponding port on the TaqMan Array.

ORGANISM(S): Homo sapiens

SUBMITTER: Kehinde Ross

PROVIDER: E-GEOD-41838 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Expression of microRNA-184 in keratinocytes represses argonaute 2.

Roberts Julian C JC Warren Richard B RB Griffiths Christopher E M CE Ross Kehinde K

Journal of cellular physiology 20131201 12

Interleukin-22 (IL-22) is a proinflammatory cytokine that has been associated with the pathogenesis of inflammatory skin disorders. However, the impact of IL-22 on microRNA (miRNA) expression in epidermal keratinocytes is unknown. Here we show that IL-22 induces miR-184 in reconstituted human epidermis (RHE) and in the HaCaT keratinocyte cell line. Exposure to IL-22 increased miR-184 expression 8- and 15-fold in RHE and HaCaT cells, respectively. Oncostatin M, an unrelated proinflammatory cytoki ...[more]

PMID: 23696368

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Dataset Information

Real-time quantitative PCR analysis of microRNA in keratinocytes and reconstituted epidermis

Publications

Expression of microRNA-184 in keratinocytes represses argonaute 2.

Similar Datasets

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