Transcriptome profiling of wild type primordial germ cells (PGC) by RNAseq

ABSTRACT: Purpose: To identify molecular pathways underlying epigenetic reprogramming in early germ cell precursors, we examined global gene expression of wild type primordial germ cells using mRNA sequencing. Methods: Given the limited number of PGCs collected from E9.5 to E13.5 (ranging from 300 to 5000), we used a low-input RNA sequencing method, Smart-Seq. RNA libraries were pooled and sequenced by Illumina Hiseq. Results: We generated >22 million uniquely mapped reads per sample and identified >10 thousand transcripts per genotype (RPKM>0.1). Hierarchical clustering and correlation analysis on gene expression indicates samples were clearly separated according to their genotypes with Spearman correlation coefficient of 0.98/0.99 within biological replicates. Compared with E9.5 PGCs, 479 genes we

ORGANISM(S): Mus musculus

SUBMITTER: Dinh Diep 

PROVIDER: E-GEOD-41908 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress