IL-7 and IL-15 instruct the generation of human memory stem T cells from naïve precursors

ABSTRACT: The identification of the most appropriate T-cell subset to ensure optimal persistence and anti-tumor activity is a major goal of cancer immunotherapy. We identified a novel post-mitotic CD45RA+CD62L+ T cell subpopulation (TTN), generated in vitro upon activation of naïve T (TN) cells with beads conjugated to anti-CD3 and anti-CD28 antibodies. This cell population is highly proliferative, produces low levels of IFNg and cytotoxic molecules, and requires IL-7 and IL-15 for in vitro expansion. To investigate whether this cell population represents a novel T-cell subset, we compared the transcriptomic profile of TTN with that of other T-cell subsets, namely naturally occurring TN and central memory (TCM) and manipulated cells of TCM origin (TTCM). We collected RNA from unmanipulated purified

ORGANISM(S): Homo sapiens

SUBMITTER: Silvio Bicciato 

PROVIDER: E-GEOD-41909 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress