Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Effects of exercise on gene expression level in human NK Cells

ABSTRACT: We tested the hypothesis on the mechanisms responsible for the early control of NK cell function by identifying a discrete set of genes in circulating NK cells that were altered by exercise. Exercise leads to a rapid change in the profile of gene expression in NK cells. Twelve healthy men (20-29 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 77% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACSM-BM-. Pro Separator). Total RNA was extracted using TRIzolM-BM-.. For this study we used Affymetrix plus 2 (total of 24 chips).

ORGANISM(S): Homo sapiens

SUBMITTER: Shlomit Radom-Aizik

PROVIDER: E-GEOD-41914 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Impact of brief exercise on peripheral blood NK cell gene and microRNA expression in young adults.

Radom-Aizik Shlomit S Zaldivar Frank F Haddad Fadia F Cooper Dan M DM

Journal of applied physiology (Bethesda, Md. : 1985) 20130103 5

Natural killers (NK) cells are unique innate immune cells that increase up to fivefold in the circulating blood with brief exercise and are known to play a key role in first-response defense against pathogens and cancer immunosurveillance. Whether exercise alters NK cell gene and microRNA (miRNA) expression is not known. Thirteen healthy men (20-29 yr old) performed ten 2-min bouts of cycle ergometer exercise at a constant work equivalent to an average of 77% of maximum O2 consumption interspers ...[more]

PMID: 23288554

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Dataset Information

Effects of exercise on gene expression level in human NK Cells

Publications

Impact of brief exercise on peripheral blood NK cell gene and microRNA expression in young adults.

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