Accelerated growth in the absence of DNA replication origins

ABSTRACT: DNA replication initiates at defined sites called origins, which serve as binding sites for initiator proteins that recruit the replicative machinery. Origins differ in number and structure across the three domains of life1 and their properties determine the dynamics of chromosome replication. Bacteria and some archaea replicate from single origins, whilst most archaea and all eukaryotes replicate using multiple origins. Initiation mechanisms that rely on homologous recombination operate in some viruses. Here we show that such mechanisms also operate in archaea. We have used deep sequencing to study replication in Haloferax volcanii. Four chromosomal origins of differing activity were identified. Deletion of individual origins resulted in perturbed replication dynamics and reduced growth.

ORGANISM(S): Haloferax volcanii

SUBMITTER: Conrad Nieduszynski 

PROVIDER: E-GEOD-41961 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress