Gene expression in a three-dimensionally cultivated human hepatocellular carcinoma (HCC) model

ABSTRACT: The development of more complex but reliable systems for compound testing in a pharmaceutical context is a challenging task to date. Three-dimensional (3D), organ mimetic cell culture is aiming to become an alternative to common two-dimensional (2D) cell culture or animal testing in that field. We developed a biocompatible 3D cell culture environment for a hepatocellular carcinoma (HCC) model that enables cellular maintenance in a polycarbonate scaffold structure. Albumin, regarded as a differentiation marker, was elevated in statically 3D cultivated HepG2 cells. Expression of HCC tumor marker alpha-fetoprotein (AFP) was reduced compared to immunofluorescence stainings of 2D cultivated cells. Remarkably, expression of cytokeratin and pathophysiologically relevant beta-1 integrin (ITGB1) wa

ORGANISM(S): Homo sapiens

SUBMITTER: Uta Fernekorn 

PROVIDER: E-GEOD-41962 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress