Transcriptomic Analysis of PNN- and ESRP1-regulation in Human Corneal Epithelial Cells

ABSTRACT: Pnn depletion in developing mouse corneal epithelium led to disrupted alternative splicing of multiple ESRP-regulated epithelial-type exons. In human corneal epithelial cells (HCET), ESRP1 and PNN displayed close localization in and around nuclear speckles and their physical association in protein complexes was identified. In this study, gene expression profiling was performed to identify PNN- and ESRP1-regulated alternative pre-mRNA splicing in human corneal epithelial cells. Immortalized human corneal epithelial cells harboring doxycycline-inducible shRNA against PNN or ESRP1 were created. Whole transcriptome array analysis on ESRP1 or PNN knockdown HCET cells revealed clear alterations in transcript level and splicing pattern of specific subsets of genes with significant overlaps in t

ORGANISM(S): Homo sapiens

SUBMITTER: Jian-Liang Li 

PROVIDER: E-GEOD-41996 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress