Transcription profiling of Zebrafish embryos investigating the effect of miR-430 microRNA on deadenylation and clearance of maternal mRNAs

ABSTRACT: MicroRNAs comprise 1-3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. Using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target mRNAs. Targets are highly enriched for maternal mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis. Experiment Overall Design: As a first step towards the identification of miR-430 targets, we compared the rate of mRNA degradation in

ORGANISM(S): Danio rerio

SUBMITTER: Antonio Giraldez 

PROVIDER: E-GEOD-4201 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress