Steady-state samples of Methanococcus maripaludis MM901 grown on formate under hydrogen limited conditions

ABSTRACT: Hydrogenotrophic methanogenic Archaea are defined by a H2 requirement for growth. Despite this requirement, many hydrogenotrophs are also capable of growth with formate as an electron donor for methanogenesis. Hydrogenotrophs respond to H2 starvation both phenotypically and at the level of gene expression; however, the responses during growth on formate have not been characterized. Here we report that during continuous culture of Methanococcus maripaludis under defined nutrient conditions, growth yields relative to methane production decreased markedly with either H2 excess or formate excess, suggesting that energy spilling occurs. Using microarray analysis, we show that the expression of genes encoding F420-dependent steps of methanogenesis, including one of two formate dehydrogenases, in

ORGANISM(S): Methanococcus maripaludis S2

SUBMITTER: Sung Ho Yoon 

PROVIDER: E-GEOD-42111 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress