Restoration and reinforcement of acclimatizing responses during the adaptation of an unfit, metabolically engineered strain

ABSTRACT: Organisms cope with physiological stressors through acclimatizing mechanisms in the short-term, and through adaptive mechanisms over evolutionary timescales. Whereas the former offer a consistent and largely predictable buffer against stressors, myriad paths of adaptation are often possible. Our work examined whether knowledge of acclimatizing responses could be informative ofM-BM- aspects of future adaptation, using as a model system a strain of Methylobacterium extorquens AM1 that was experimentally engineered and then evolved with a novel central metabolism. The engineered strain is markedly slower and less fit than wild-type, which is reflected in microarray analyses by hundreds of genes with differential expression, and also altered levels NAD(P)(H) metabolites. Yet after 600 generat

ORGANISM(S): Methylobacterium extorquens AM1

SUBMITTER: Sean Carroll 

PROVIDER: E-GEOD-42116 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress