Precise Maps of RNA Polymerase Reveal How Promoters Direct Initiation and Pausing

ABSTRACT: Transcription regulation occurs frequently through promoter-associated pausing of RNA polymerase II (Pol II). We developed a Precision nuclear Run-On and sequencing assay (PRO-seq) to map the genome-wide distribution of transcriptionally-engaged Pol II at base-pair resolution. Pol II accumulates immediately downstream of promoters, at intron-exon junctions that are efficiently used for splicing, and over 3' poly-adenylation sites. Focused analyses of promoters reveal that pausing is not fixed relative to initiation sites nor is it specified directly by the position of a particular core promoter element or the first nucleosome. Core promoter elements function beyond initiation, and when optimally positioned they act collectively to dictate the position and strength of pausing . We test this

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Hojoong Kwak 

PROVIDER: E-GEOD-42117 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress