Steady-state samples of Methanococcus maripaludis MM901:del_fruA (MMP1382)-del_frcA (MMP0820)

ABSTRACT: Effects of fruA (MMP1382) and frcA (MMP0820) on physiology and regulation of methanogenesis in Methanococcus maripaludis The strain was grown by continuous culture in a one-liter fermenter (New Brunswick Scientific, Edison, NJ) at 37M-BM-0C (FEMS Microbiol Lett 238: 85-91, 2004). Medium and gas compositions were modified from those for non-limiting conditions (BMC Microbiol 9: 149, 2009). The standard gassing regime was 110 mL/min H2, 40 mL/min CO2, 35 mL/min Ar, and 15 mL/min H2S/Ar mixture (1:99). Hydrogen limited condition was 22 mL/min H2. After the OD increased above 0.6 (24 h), the medium flow was turned on at 0.083 L/h. Medium was either phosphate limiting (0.12 mM K2HPO4) or phosphate excess (0.8 mM K2HPO4). Culture samples (1.5 mL) were rapidly removed from the vessels by syringe

ORGANISM(S): Methanococcus maripaludis S2

SUBMITTER: Sung Ho Yoon 

PROVIDER: E-GEOD-42122 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress