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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profile in melanomas from Pdk1-inactivated BrafV600E::Pten-/- mice

ABSTRACT: Gene expression profiling was performed to access the changes in gene expression in melanomas from Pdk1-inactivated Brafv600E::Pten-/- mice. The expression profiles of the BrafV600E::Pten-/-::Pdk1-/- were compared to the BrafV600E::Pten-/-::Pdk+/+ genotypes. The analysis has identified several important signaling pathways in Pdk1-dependent melanomagenesis. Melanoma tumors from the BrafV600E::Pten-/-::Pdk1+/+ and BrafV600E::Pten-/-::Pdk1-/- genotypes were harvested and mRNA from each group was pooled to enable four biologically replicates analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Jian-Liang Li

PROVIDER: E-GEOD-42187 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genetic inactivation or pharmacological inhibition of Pdk1 delays development and inhibits metastasis of Braf(V600E)::Pten(-/-) melanoma.

Scortegagna M M Ruller C C Feng Y Y Lazova R R Kluger H H Li J-L JL De S K SK Rickert R R Pellecchia M M Bosenberg M M Ronai Z A ZA

Oncogene 20130916 34

Phosphoinositide-dependent kinase-1 (PDK1) is a serine/threonine protein kinase that phosphorylates members of the conserved AGC kinase superfamily, including AKT and protein kinase C (PKC), and is implicated in important cellular processes including survival, metabolism and tumorigenesis. In large cohorts of nevi and melanoma samples, PDK1 expression was significantly higher in primary melanoma, compared with nevi, and was further increased in metastatic melanoma. PDK1 expression suffices for i ...[more]

PMID: 24037523

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Gene expression profile in melanomas from Pdk1-inactivated BrafV600E::Pten-/- mice

Project description:Gene expression profiling was performed to access the changes in gene expression in melanomas from Pdk1-inactivated Brafv600E::Pten-/- mice. The expression profiles of the BrafV600E::Pten-/-::Pdk1-/- were compared to the BrafV600E::Pten-/-::Pdk+/+ genotypes. The analysis has identified several important signaling pathways in Pdk1-dependent melanomagenesis.

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mTORC1 activation blocks BrafV600E-induced growth-arrest but is insufficient for melanoma formation

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Beta-catenin Controls Metastasis in Braf-activated Pten-inactivated Melanomas

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Pigment producing melanocytes as the cells of origin of BRAFV600E-induced mouse cutaneous melanoma

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