Leishmania donovani amastigotes: Nelfinavir (NFV)-sensitive (control) vs Nelfinavir-resistant [Comparative Genomic Hybridization (DNA) profiling]

ABSTRACT: Drug resistance is a major public health challenge in Leishmaniasis chemotherapy, particularly in the case of emerging Leishmania/HIV-1 co-infections. Recently, we have delineated the mechanism of cell death induced by the HIV-1 protease inhibitor, Nelfinavir, in the Leishmania parasite. In order to investigate the underlying molecular mechanism involved in Nelfinavir resistance, in vitro Nelfinavir resistant amastigotes were developed by direct drug pressure in culture. RNA expression profiling analyses of closely related Leishmania species were used as a screening tool to compare Nelfinavir-resistant and -sensitive parasites in order to identify candidate genes involved in drug resistance, and several genes were found to be differentially expressed. Comparative gene hybridization (CGH) a

ORGANISM(S): Leishmania donovani

SUBMITTER: Frederic Raymond 

PROVIDER: E-GEOD-42195 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress