Studying Gene Expression Profiles of UV-Irradiated Human Keratinocytes by cDNA Microarray

ABSTRACT: The goal of this thesis is to study the response of a human keratinocyte cell line (HaCaT cells) after UVB irradiation. In order to develop a system for UV irradiation, we firtstly studied the UV illumination system to find out a stable and reliable condition for UV irradiation experiments. Further, we found out the proper dose of UVB radiation and time points after UVB irradiation by performing MTT assay and trypan blue viability test. According to the results of MTT assay and trypan blue viability test, the cellular activity as well as survival rate of UVB-irradiated cells were in proportion to the radiation doses within a lower UVB dose range. We wanted to find out the possible reasons for this phenomenon by using cDNA microarray. We hope the thesis could be a guide for the following re

ORGANISM(S): Homo sapiens

SUBMITTER: ChengWei Chang 

PROVIDER: E-GEOD-4223 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress