Transcription profiling of THP-1 cells reveals nitric oxide activation of Erk regulates the stability and translation of mRNA transcripts containing CU-rich elements

ABSTRACT: Nitric oxide (NO) can stabilize mRNA by activating p38 mitogen-activated protein kinase (MAPK). Here, transcript stabilization by NO was investigated in human THP-1 cells using microarrays. After LPS pre-stimulation, cells were treated with actinomycin D and then exposed to NO without or with the p38 MAPK inhibitor SB202190. The decay of 220 mRNAs was affected; most were stabilized by NO. Unexpectedly, SB202190 often enhanced rather than antagonized transcript stability. NO activated p38 MAPK and Erk1/2; SB202190 blocked p38 MAPK, but further activated Erk1/2. PCR confirmed that NO and SB202190 could additively stabilize mRNA, an effect abolished by Erk1/2 inhibition. In affected genes, these responses were associated with CU-rich elements (CURE) in 3 un-translated regions. NO stabilize

ORGANISM(S): Homo sapiens

SUBMITTER: Shuibang Wang 

PROVIDER: E-GEOD-4228 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress