Depth Distribution of Ammonia Oxidation Rates and Ammonia-Oxidizer Community Composition in the Sargasso Sea
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ABSTRACT: Ammonia oxidizer community structure were examined in a depth profile from 20 to 2000 m at the Bermuda Atlantic Time-series Study using a functional gene microarray to look at amoA diversity Two color array (cy3 and cy5): the universal standard 20 bp oligo (fluoresced with cy5) is printed to the slide with a 70-mer. Environmental DNA sequences (fluoresced with Cy3) within 15% of the 70-mer will bind to it. Signal is the cy3/cy5. Two replicate arrays were run on duplicate targets.
ORGANISM(S): synthetic construct
SUBMITTER: Silvia Newell
PROVIDER: E-GEOD-42287 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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