Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression analysis of Gordonia sp. KTR9 and Gordonia sp. KTR9 mutant GlnR upon exposure to high and low Nitrogen conditions


ABSTRACT: Investigation of gene expression level changes in Gordonia sp. KTR9 and Gordonia sp. KTR9 mutant GlnR upon exposure to high and low nitrogen conditions The Gordonia sp. KTR9 strain used in this study has been previously described by Thompson KT, Crocker FH, Fredrickson HL.2005. Mineralization of the cyclic nitramine explosive hexahydro-1,3,5-trinitro-1,3,5-triazine by Gordonia and Williamsia spp. Appl Environ Microbiol. 2005 Dec;71(12):8265-72. A 12 x 135K array study using total RNA recovered from triplicate cultures of KTR9 exposed to high nitrogen conditions, triplicate cultures of KTR9 exposed to low nitrogen conditions, triplicate cultures of KTR9 mutant GlnR exposed to high nitrogen conditions, triplicate cultures of KTR9 mutant GlnR exposed to low nitrogen conditions.

ORGANISM(S): Gordonia sp. KTR9

SUBMITTER: Dawn Hancock 

PROVIDER: E-GEOD-42341 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Role of nitrogen limitation in transformation of RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) by Gordonia sp. strain KTR9.

Indest Karl J KJ   Hancock Dawn E DE   Jung Carina M CM   Eberly Jed O JO   Mohn William W WW   Eltis Lindsay D LD   Crocker Fiona H FH  

Applied and environmental microbiology 20121228 5


The transcriptome of RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine)-degrading strain Gordonia sp. strain KTR9 and its glnR mutant were studied as a function of nitrogen availability to further investigate the observed ammonium-mediated inhibition of RDX degradation. The results indicate that nitrogen availability is a major determinant of RDX degradation and xplA gene expression in KTR9. ...[more]

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